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KMID : 0880220120500050874
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Journal of Microbiology
2012 Volume.50 No. 5 p.874 ~ p.877
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Construction and characterisation of an antifungal recombinant Bacillus thuringiensis with an expanded host spectrum
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Qin Liu
Roh Jong-Yul
Yong Wang
Choi Jae-Young
Xue Ying Tao
Je Yeon-Ho
Kim Jae-Su
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Abstract
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A novel antifungal Bacillus thuringiensis strain 19?22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.
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KEYWORD
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Bacillus thuringiensis, antifungal activity, cry1E, insecticidal activity
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